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Home > Protocols

Protocols

Genetics

Quarantine procedure
Neomycin selection
Retrospective genotyping (by PCR, lethality, or infertility)
Inverse PCR (Protocol from GDP)

Cell Biology

Standard ovary dissections and immunofluorescence
Ovary dissection and staining data sheet
Optimizing fixation for a new antibody
pERK staining in the germarium

Computational resources

How to connect to the server

Molecular Biology and Biochemistry

DNA extraction for single fly PCR
Bradford assay for protein quantitation
Western Blot

Links

DSCB Program

Tetrad Program

BMS Program

PROPEL Program

PREP Program

Anatomy Department

Center for Reproductive Sciences

Broad Center for Regenerative Medicine and Stem Cell Research

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