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Protocols

Genetics

• Quarantine procedure

• Neomycin selection

• Retrospective genotyping (by PCR, lethality, or infertility)

• Stem cell replacement assay

• Bloomington Database (updated Aug 8th, 2010)

Cell Biology

• Standard ovary dissections and immunofluorescence

• Optimizing fixation for a new antibody

Molecular Biology

• DNA extraction for single fly PCR

• RNA extraction

Biochemistry

• Bradford assay for protein quantitation

• Western

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